abstract：OBJECTIVES:Breast cancer is the most common malignancy among Egyptian women. The aim of this study is to evaluate the role of both telomerase and TPS estimation in assessment of breast cancer. METHODS:The study included 40 patients with breast cancer, and 20 patients with benign breast diseases. Telomerase activity in...

journal_title：Clinical biochemistry

authors： Kassim SK,Fawzy H,El Rassad MM,Khalil FK,Khalifa A

更新日期：2001-09-01 00:00:00

abstract：:Increasing interest in the use of tumor markers in the clinical management of cancer patients has encouraged development of guidelines by local, national and international groups. Such guidelines generally include recommendations about which markers are likely to be most helpful in given circumstances. Particular requ...

journal_title：Clinical biochemistry

authors： Sturgeon CM

更新日期：2001-07-01 00:00:00

abstract：BACKGROUND:The p53 gene is an established tumor suppressor and an inducer of apoptosis. We here attempt to determine whether the putative anticarcinogenic properties attributed to red wine and its polyphenolic constituents depend, at least in part, upon their ability to modulate p53 expression in cancer cells. METHODS...

journal_title：Clinical biochemistry

authors： Soleas GJ,Goldberg DM,Grass L,Levesque M,Diamandis EP

更新日期：2001-07-01 00:00:00

abstract：OBJECTIVE:Effects of metamizol and magnesium sulfate on erythrocyte glucose 6-phosphate dehydrogenase enzyme activity were investigated in in vitro and in vivo conditions. METHODS:For in vitro studies, glucose 6-phosphate dehydrogenase was purified from human erythrocyte and rats were used for in vivo studies. Enzyme ...

journal_title：Clinical biochemistry

authors： Ciftçi M,Ozmen I,Büyükokuroğlu ME,Pençe S,Küfrevioğlu OI

更新日期：2001-06-01 00:00:00

abstract：OBJECTIVES:To evaluate whether caspases are involved in ethanol (EtOH)-induced apoptosis and if polyenylphosphatidylcholine (PPC) affects apoptosis, in vitro in Hep G2 cells. METHODS:Cells were treated with 100 mmol/L EtOH for 24 h and with 2 doses of 100 mmol/L EtOH (1/24 h) in the presence of absence of 20 mmol/L of...

journal_title：Clinical biochemistry

authors： Katz GG,Shear NH,Malkiewicz IM,Valentino K,Neuman MG

更新日期：2001-05-01 00:00:00

abstract：OBJECTIVES:To evaluate the association between plasma lipids and insulin and variation in the genes for apolipoproteins (APO) E (CfoI), B (insertion/deletion), C1 (HpaI), and C3 (C-482T, C3238G) in a population-based Czech Slavonic study. DESIGN AND METHODS:In 131 men and 154 women, polymorphisms were investigated usi...

journal_title：Clinical biochemistry

authors： Hubacek JA,Waterworth DM,Poledne R,Pitha J,Skodová Z,Humphries SE,Talmud PJ

更新日期：2001-03-01 00:00:00

abstract：:Mycophenolate mofetil is widely used in combination with either cyclosporine or tacrolimus for rejection prophylaxis in renal and heart transplant patients. Although not monitored routinely nearly to the degree that other agents such as cyclosporine or tacrolimus, there is an expanding body of experimental evidence fo...

journal_title：Clinical biochemistry

authors： Shaw LM,Korecka M,DeNofrio D,Brayman KL

更新日期：2001-02-01 00:00:00

abstract：OBJECTIVES:Although monitoring of cyclosporin (CsA) is standard clinical practice postrenal transplantation, mycophenolic acid (MPA) concentrations are not routinely measured. There is evidence that a relationship exists between MPA area under the concentration-time curve (AUC) and rejection. In this study, a retrospec...

journal_title：Clinical biochemistry

authors： Pillans PI,Rigby RJ,Kubler P,Willis C,Salm P,Tett SE,Taylor PJ

更新日期：2001-02-01 00:00:00

abstract：OBJECTIVES:Carbohydrate-deficient transferrin CDT has originally been defined as the sum of isotransferrins exhibiting isoelectric point values > or = 5.7 asialo, monosialo, and disialo transferrin but may also include at least in part trisialo transferrin when measured by modern commercial immunoassays. To examine the...

journal_title：Clinical biochemistry

authors： Lipkowski M,Dibbelt L,Seyfarth M

更新日期：2000-11-01 00:00:00

abstract：OBJECTIVES:The plasma apolipoprotein B (apo B) concentrations have been considered to be a more accurate representation of atherogenic particles and it has been proposed that the formula LDL-C (mmol/L) = 0.41TC - 0.32TG + 1.70apo B - 0.27 is reliable for the estimation of LDL-C (Clin Chem 1997; 43: 808-15). We undertoo...

journal_title：Clinical biochemistry

authors： Bairaktari E,Hatzidimou K,Tzallas C,Vini M,Katsaraki A,Tselepis A,Elisaf M,Tsolas O

更新日期：2000-10-01 00:00:00

abstract：OBJECTIVES:To evaluate the results of oxygen consumption measurement in lymphocytes for the diagnosis and treatment monitoring of pediatric patients with oxidative phosphorylation diseases. DESIGN AND METHODS:Twenty-four children with an oxidative phosphorylation disease were studied. Results were compared with those ...

journal_title：Clinical biochemistry

authors： Artuch R,Colomé C,Playán A,Alcaine MJ,Briones P,Montoya J,Vilaseca MA,Pineda M

更新日期：2000-08-01 00:00:00

abstract：OBJECTIVES:It has recently been suggested that when adjusting doses of cyclosporine (CsA), determining its concentration in blood samples taken 2 h postdose (C(2)) is more clinically beneficial than using the predose concentration (C(0)). We determined C(0) and C(2) concentrations of CsA and their metabolites in sample...

journal_title：Clinical biochemistry

authors： Fernández-Marmiesse A,Hermida J,Tutor JC

更新日期：2000-07-01 00:00:00

abstract：OBJECTIVES:To assess the relationship between lipoprotein (a) [Lp (a)] and lipoprotein oxidation in patients with coronary artery disease (CAD). DESIGN AND METHODS:Oxidation of apolipoprotein (apo)B-containing lipoproteins, vitamin E, carotenoids, lipid-lipoprotein levels were determined in 171 CAD and 70 non-CAD subj...

journal_title：Clinical biochemistry

authors： Serdar Z,Sarandöl E,Dirican M,Yeşilbursa D,Serdar A,Tokullugil A

更新日期：2000-06-01 00:00:00

abstract：OBJECTIVES:The purpose of this study was to measure the extent of lipid peroxidation and the status of antioxidants in patients with Hodgkin's disease. DESIGN AND METHODS:Glutathione peroxidase (GPX) and superoxide dismutase (SOD) activities, and malondialdehyde (MDA), selenium, zinc and copper content have been measu...

journal_title：Clinical biochemistry

authors： Güven M,Oztürk B,Sayal A,Ozet A

更新日期：2000-04-01 00:00:00

abstract：OBJECTIVES:Characterization of beta-galactosidase in leukocytes and fibroblasts of heterozygotes for GM1 type I. DESIGN AND METHODS:Leukocyte and fibroblast beta-galactosidase activity was determined fluorimetrically using 4-methylumbelliferyl-beta-D-galactoside as an artificial substrate. Optimum pH, Km, Vmax and the...

journal_title：Clinical biochemistry

authors： Sopelsa AM,Severini MH,Da Silva CM,Tobo PR,Giugliani R,Coelho JC

更新日期：2000-03-01 00:00:00

abstract：OBJECTIVES:We have previously identified three minor immunophilins of molecular weights 37 kDa, 14 kDa, and 5-8 kDa capable of binding tacrolimus and sirolimus. DESIGN AND METHODS:When tested against pure preparations of five sirolimus metabolites, the 14 kDa protein had almost no cross-reactivity, the 37 kDa protein ...

journal_title：Clinical biochemistry

authors： Davis DL,Murthy JN,Gallant-Haidner H,Yatscoff RW,Soldin SJ

更新日期：2000-02-01 00:00:00

abstract：BACKGROUND:The aim of this study was to investigate whether BGD activity is of additional value in the assessment of pulmonary inflammation caused by coal dust exposure. DESIGN AND METHODS:Ex-coalminers were included in this study. Forty-eight healthy male subjects, without a relevant medical history, were used as con...

journal_title：Clinical biochemistry

authors： Cobben NA,Drent M,De Vries J,Wouters EF,Van Dieijen-Visser MP,Henderson RF

更新日期：1999-11-01 00:00:00

abstract：OBJECTIVES:The liver has tremendous regenerative capacity but can be damaged by toxins, such as ethanol (EtOH). It has long been known that EtOH inhibits liver regeneration. Recent work demonstrates that the proinflammatory cytokine, tumor necrosis factor ( (TNF), is required for normal liver regeneration, as well as f...

journal_title：Clinical biochemistry

authors： Diehl AM

更新日期：1999-10-01 00:00:00

abstract：OBJECTIVES:To ascertain the effects of eating on plasma antioxidant capacity in patients with liver disease. DESIGN AND METHODS:Eighteen cirrhotic patients were compared to 18 age and sex-matched controls. TRAP was measured by a fluorometric assay after a 12 h fast, and 60, 120, and 180 min after the study participant...

journal_title：Clinical biochemistry

authors： Pirisi M,Federico E,Bortolotti N,Fabris C,Falleti E,Soardo G,Gonano F,Bartoli E

更新日期：1999-08-01 00:00:00

abstract：OBJECTIVES:Studies from both experimental animals and humans suggest that administration of exogenous DHEA may have beneficial endocrine-metabolic, immunologic and neurologic effects. Several groups have administered DHEA to humans, but to the best of our knowledge, no one at this point has published a summary of the r...

journal_title：Clinical biochemistry

authors： Tummala S,Svec F

更新日期：1999-07-01 00:00:00

abstract：OBJECTIVE:Apolipoprotein E (APOE) E4, apolipoprotein B-100 (APOB) Q3611 allele, the angiotensin converting enzyme (ACE) deletion (D) allele and glycoprotein IIIa (GP3A) P33 mutant allele are reported to predispose to early-onset coronary heart disease (CHD). These associations were not all confirmed in more recent stud...

journal_title：Clinical biochemistry

authors： Nassar BA,Dunn J,Title LM,O'Neill BJ,Kirkland SA,Zayed E,Bata IR,Cantrill RC,Johnstone J,Dempsey GI,Tan MH,Breckenridge WC,Johnstone DE

更新日期：1999-06-01 00:00:00

abstract：OBJECTIVE:Erythrocyte pyrimidine 5'-nucleotidase (Pyr-5'-N) is highly sensitive to heavy metal inactivation in vitro and in vivo, and a number of studies have verified its usefulness as a biomarker of acute and chronic lead exposures. Retrospective and prospective studies attempted to determine whether the known linear...

journal_title：Clinical biochemistry

authors： Paglia DE,Renner SW,Bhambhani K

更新日期：1999-04-01 00:00:00

abstract：OBJECTIVES:To investigate the association between oxidative stress and certain trace elements in the blood of breast cancer patients. DESIGN AND METHODS:Malondialdehyde (MDA) was measured in serum of patients with breast cancer (n = 35) and controls (n = 35) by high performance liquid chromatography. Trace elements we...

journal_title：Clinical biochemistry

authors： Huang YL,Sheu JY,Lin TH

更新日期：1999-03-01 00:00:00

abstract：OBJECTIVE:To quantify pepsinogen C (PEPC) and prostaglandin D synthase (PGDS) in breast cyst fluid and examine if these two parameters can be used for breast cyst type classification. DESIGN AND METHODS:We quantified PEPC and PGDS in 92 and 50 breast cyst fluids, respectively, using previously established immunofluoro...

journal_title：Clinical biochemistry

authors： Borchert GH,Melegos DN,Yu H,Giai M,Roagna R,Ponzone R,Sgro L,Diamandis EP

更新日期：1999-02-01 00:00:00

abstract：OBJECTIVE:We evaluated the erythrocyte lipid susceptibility to oxidation and erythrocyte antioxidant status in patients with myocardial infarction (MI). DESIGN AND METHODS:Patients with MI were divided into two group according to the severity of the disease as severe (n = 30) or milder (n = 25). Malondialdehyde as a m...

journal_title：Clinical biochemistry

authors： Konukoğlu D,Akçay T,Erdem T

更新日期：1998-11-01 00:00:00

abstract：INTRODUCTION:In the Olympus uric acid procedure, uric acid is converted by uricase to allantoin and hydrogen peroxide, which is reacted in a Trinder reaction to produce a chromophore read bichromatically at 520 and 660 nm. Repeated difficulty was encountered in obtaining uric acid results on samples from myeloma patien...

journal_title：Clinical biochemistry

authors： Langman LJ,Allen LC,Romaschin AD

更新日期：1998-10-01 00:00:00

abstract：OBJECTIVES:The International Federation of Clinical Chemistry (IFCC) initiated a pilot study, with cortisol as an example, that aims to implement the concept of standardization of hapten immunoprocedures on the basis of metrological traceability. In fact, this standardization concept comes down to correct calibration o...

journal_title：Clinical biochemistry

authors： Thienpont LM

更新日期：1998-08-01 00:00:00

abstract：OBJECTIVE:This study aimed at evaluating Elecsys free triiodothyronine (FT3) assay performed on an Elecsys 2010 system, while paying special attention to age relationship in euthyroid subjects. DESIGN AND METHODS:FT3 levels were measured in 149 euthyroid control subjects aged between 2 and 92 years old, 33 hyperthyroi...

journal_title：Clinical biochemistry

authors： Sapin R,Schlienger JL,Goichot B,Gasser F,Grucker D

更新日期：1998-07-01 00:00:00

abstract：OBJECTIVE:A survey of operators of a bedside blood glucose monitoring (BGM) program at a tertiary health care institution was performed to identify potential outcome indicators for our quality assurance program. DESIGN AND METHODS:170 surveys were randomly distributed to each nursing unit. The survey consisted of 20 q...

journal_title：Clinical biochemistry

authors： Collier CP,Houlden RL,Gleeson KA,Patrzykat A,Rhymer SL

更新日期：1998-06-01 00:00:00

abstract：OBJECTIVE:We performed a side-by-side comparison between three stain-then-lyse commercially available methods (Ortho-mune Lysing solution, FACS lysing solution and ImmunoPrep reagent system) and a lyse-then-stain method using hypotonic NH4Cl. The major difference between these methods is that only in the latter the ali...

journal_title：Clinical biochemistry

authors： Pacifici R,Zuccaro P,Cozzi-Lepre A,di Carlo S,Bacosi A,Fattorossi A

更新日期：1998-04-01 00:00:00

abstract：OBJECTIVES:We evaluated the analytical performance of the Elecsys 2010 immunoanalyzer (Boehringer Mannheim Canada), which is based on a new detection technology, electrochemical luminescence. DESIGN AND METHODS:We used six representative assays from the initial launch menu of the instrument: thyroid stimulating hormon...

journal_title：Clinical biochemistry

authors： Forest JC,Massé J,Lane A

更新日期：1998-03-01 00:00:00

abstract：OBJECTIVES:To evaluate the analytical performance of the testosterone assay performed on the Bayer immuno 1 system from Bayer Corporation. DESIGN AND METHODS:The precision was measured using three Bayer TESTpoint Ligand controls, three Medical Decision Pools and the Bayer SETpoint Testosterone calibrators. The lineari...

journal_title：Clinical biochemistry

authors： Lévesque A,Letellier M,Swirski C,Lee C,Grant A

更新日期：1998-02-01 00:00:00

abstract：OBJECTIVES:An overview of biochemical markers of bone metabolism is presented along with indications for their clinical utilization. DESIGN AND METHODS:The structure, cyclical metabolism, and hormone regulation of bone is reflected by markers of resorption, formation and/or turnover. Markers of resorption representing...

journal_title：Clinical biochemistry

authors： Christenson RH

更新日期：1997-12-01 00:00:00

abstract：OBJECTIVES:We studied the stability of maternal blood markers for screening for Down syndrome (alpha-fetoprotein, unconjugated estriol, intact human chorionic gonadotropin (hCG) and free beta-human chorionic gonadotropin) upon repeated freeze-thaw cycles. DESIGN AND METHODS:Forty-three samples collected from second tr...

journal_title：Clinical biochemistry

authors： Massé J,Forest JC,Moutquin JM,Degrandpre P

更新日期：1997-10-01 00:00:00

abstract：OBJECTIVE:To establish a plasma calibration curve for the Advantage Blood Glucose System that would permit direct comparison with results provided by clinical laboratories. DESIGN AND METHODS:The initial study involved the comparison between the Advantage whole blood referenced results and the laboratory plasma result...

journal_title：Clinical biochemistry

authors： Lane A,Geadah D,Laferriere M,Lebrun M,Caron D,Ross P

更新日期：1997-08-01 00:00:00

abstract：OBJECTIVES:To develop immunofluorometric procedures for measuring BRCA1 protein in various biological fluids and tissue extracts. DESIGN AND METHODS:Five commercially available monoclonal and polyclonal antibodies against BRCA1 were evaluated for developing competitive and non-competitive immunofluorometric procedures...

journal_title：Clinical biochemistry

authors： Lianidou ES,Melegos DN,Diamandis EP

更新日期：1997-07-01 00:00:00

abstract：OBJECTIVE:The purpose of this study was to develop an improved method of direct DNA sequencing, which makes it possible to identify heterozygous mutations of the lipoprotein lipase (LPL) gene in order to understand the underlying genetic disorder of type IV hyperlipoproteinemia. METHODS AND RESULTS:The direct sequenci...

journal_title：Clinical biochemistry

authors： Mori A,Takagi A,Ikeda Y,Yamamoto A

更新日期：1997-06-01 00:00:00

abstract：OBJECTIVE:To review biochemical literature and determine the significance of non-covalent bonding in plasma. RESULTS:Many non-covalent bonds exist in plasma. Relatively strong self-associations are found for serum albumin, several gamma-globulins, apolipoproteins and fibrin(ogen). Here, fibrinogen and fibrin, consider...

journal_title：Clinical biochemistry

authors： London M

更新日期：1997-03-01 00:00:00

abstract：OBJECTIVE:Quantitative sweat production and -ionic composition in Essential Hyperhidrosis (EH), and the effects of T2-T3 thoracoscopic sympathicolysis (TS) hereon, are unknown. Standardised pilocarpine iontophoresis sweat tests were performed before and after TS in order to study these issues. DESIGN AND METHODS:Piloc...

journal_title：Clinical biochemistry

authors： Noppen M,Sevens C,Vincken WG

更新日期：1997-03-01 00:00:00

abstract：OBJECTIVES:CA 15-3 and CEA are considered useful tumor markers in monitoring breast cancer patients. This study was undertaken to specifically evaluate the transient elevations in these markers that are observed during systemic treatment for metastatic disease. This phenomenon has been termed "spiking." DESIGN AND MAT...

journal_title：Clinical biochemistry

authors： Yasasever V,Dinçer M,Camlica H,Karaloğlu D,Dalay N

更新日期：1997-02-01 00:00:00